The First Step of NGS. Attaching the Library to the Flow Cell.
Sequencing occurs on a glass surface of a flow cell. Short pieces of DNA called oligonucleotides are bound to the surface of the flow cell. These oligonucleotides match the adapter sequences of the library. First, the library gets denatured to form single DNA strands. Then this library gets added to the flow cell, which attaches to one of the two oligos. The strand that attaches to the oligo is the forward strand. Next, the reverse strand gets made, and the forward strand gets washed away. The library is now bound to the flow cell.
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