Sequencing the DNA to Create Reads.
The sequencing primer binds to the forward strands. Next, fluorescent nucleotides G, C, T and A get added to the flow cell along with DNA Polymerase. Each nucleotide has a different colour fluorescent tag and a terminator. So only one nucleotide can get sequenced at a time. First, the complementary base binds to the sequence. Then the camera reads and records the colour of each cluster. Next, a new solution flows in and removes the terminators. The nucleotides and DNA Polymerase flow in again, and another nucleotide gets sequenced. These read cycles continue for the number of reads set on the sequencer.
Watch the full video "Next Generation Sequencing - A Step-By-Step Guide to DNA Sequencing" here: 🔗 • Next Generation Sequencing - A Step-B...
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