How to perform gram staining technique practical
in this video lecture we will explain about the gram staining technique that how to perform gram staining.
1. introduction
Gram Staining is the common, important, and most used differential staining techniques in microbiology, which was introduced by Danish Bacteriologist Hans Christian Gram in 1884. This test differentiate the bacteria into Gram Positive and Gram Negative Bacteria, which helps in the classification and differentiations of microorganisms.
2. procedure
Take a clean, grease free slide.
Prepare the smear of suspension on the clean slide with a loopful of sample.
Air dry and heat fix
Crystal Violet was poured and kept for about 30 seconds to 1 minutes and rinse with water.
Flood the gram’s iodine for 1 minute and wash with water.
Then ,wash with 95% alcohol or acetone for about 10-20 seconds and rinse with water.
Add safranin for about 1 minute and wash with water.
Air dry, Blot dry and Observe under Microscope.
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